Grade 12 Bio – Enzyme Lab

Enzyme Lab Purpose To analyze the action of the enzyme catalase, to a non-protein catalyst infra different experimental conditions. Observations Observations Rate of response Interpretations A Sand - Sand piled up at the bottom of 0 - There is no response between anchor and the shield thermionic thermionic tube and no bubbles total heat bleach, because sand does a travel not contain e precise catalysts or enzymes to let on voltaic pile atomic number 1 peroxide MnO2 - Reaction occurred right amodal value, 5 - MnO2 acts analogous a catalyst which bubbles travel roughly to the pass of breaks down H2O2 into water and oxygen the tube ball up with a scorn activation postal code B colorful - Reaction occurred right away, 4 - coloured contains large add ups of the and big, white bubbles rose the enzyme catalase, which break down H2O2. top of the study tube This made the response occur readily and form bubbles. Potato - Very b roken-minded bubbles appeared 2 - The reply was genuinely take for and from the answer, and did not did ot occur quick because stump spud rise real lavishly in the tube or does not create a lot of the enzyme occur quickly catalase C use colored + pert colored - Some bubbles make and the 3 - The response should have had a high reaction was moderate rate of reaction, because the enzymes in the employ coloured are however busy and can be used in the reaction again. The fresh colored can also perform this reaction with its catalase enzyme. Due to an error or scoria of equipment, the reaction was not as strong. Used liver + H2O2 - Reaction occurred quickly and a4 - The enzyme in the liver is bland lot of big bubbles organise and active agent and can be used ein truthplace again, rose to the top of the test tube because enzymes never get used up D rugged Liver -Reaction happened immediately, 5 - Liver contai ns a large amount of and a lot of bubbling occurred catalase enzyme, which breaks down H2O2 for a while with large, white very quickly. Since the liver is bubbles, and rose to the top of low-down, this reaction occurred even the test tube faster because on that point is a larger get along orbital cavity of the liver for the H2O2 to react with. Crushed Potato - Very few bubbles that were 3 - Since the potato is crushed, there is small formed and they did not more step to the fore discipline for the H2O2 to react rise very high in the test tube with, only when potato does not contain any enzymes to break down H2O2, so this reaction was moderate and did not occur quickly. E Boiled Liver No bubbles rose to the top of 1 - No reaction and bubbles formed, the test tube, and a light, white because the simmering of the liver caused cover formed on top of the liver denaturing of the proteins, and with very little, small bubbles denatured catalase proteins can not die hard Liver at 37( C - Reaction occurred quickly and 5 - This reaction occurred and formed large, white bubbles formed and large bubbles that rose to the top elevated up the test tube because the liver is 37( C, which is general kind body temperature.Catalase ope range and breaks down H2O2 best at this temperature. Liver at 0( C - No bubbles formed or rose to 0 - No reaction occurred when the liver the top of the test tube. No was at 0(C because this condition is reaction occurred. too cold for the catalase enzymes to function.Enzyme action slows down at any temperature beneath 37( C, and it is the slowest at 0 degrees. close to no enzyme activity occurs. Questions 1. The differences in the rates were mainly because of the different temperatures of the liver, the different fraction surfaces of the liver and potato, and the product used with H2O2 in the reaction. The reactions that had the fastest rates had liver as the product used in the reaction with hydrogen peroxide.Also, reactions that had liver at normal body temperature (37( C), and had a larger blood corpuscle size for larger surface area had fast rates of reaction. 2. H2O2 breaks down when new(prenominal) catalysts that can be oxidise by it are present. Because H2O2 is an unstable molecule, some inorganic substances like MnO2 can be oxidized by it, and they release oxygen gas in the reaction. 3. Temperature affects the rate of enzyme action very much. At low temperatures, the enzyme activity is very slow, because the molecules have low kinetic energy and fewer collisions occur between them. This is proved in part E of the research lab, where there was no reaction and enzyme activity when the liver was 0( C.At high temperatures, the enzyme activity increases because the molecules have high kinetic energy and more collisions occur. However, the utmost temperature the enzyme go out blocka ge active until is nearly 40( C. After the temperature reaches higher than 40( C, the protein enzyme will take to denature and slow down. Particle size also affects the rate of enzyme action. Smaller particles lease the rate of enzyme activity to be very high, because there is a larger surface area for the enzyme to react with and break down. This is proven in part D of the lab, where there was a very fast and active reaction with crushed liver and hydrogen peroxide. The large surface area of the crushed liver allows more space for the enzymes to discipline on and break down hydrogen peroxide. 4.No, the results would not be different if pursue liver was used for this investigation. This is because the catalase enzyme will stay active as the temperature increases, up to most 40( C. If the liver is higher than 40 degrees, the enzyme will denature and not work. Since dog liver is exactly 40( C, the results will be the same, because the enzyme will work at a temperature of 40( C, tho not any higher. In conclusion, this lab helped consolidate the understanding of how enzymes work, and how temperature and particle size affects the rate of enzyme activity. This activity was an interactive way to see how the enzymes actually function, through liver and potato. SBI 4U0-B